No Panacea (yet): NGS for Small RNA

"NGS is the future", so it was said. However, the NGS-based digital expression profiling of small RNA is reported to be biased. The problem appears not to be the NGS platforms (SOLiD or Solexa), but the methods used for preparing the small RNA library.

Here is the link to the Nature Methods (July 2009) paper

http://www.nature.com/nmeth/journal/v6/n7/full/nmeth0709-474.html